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| A series of educational articles published in 'Imaging & Microscopy' that will take the reader on a journey through microscopy and digital imaging. |
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Digital Material Analysis
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The Series “Digital Materials Analysis” focuses on the areas of materials science and metallography in five chapters. It is based on the knowlege provided in the Series “Basics of Light Microscopy and Imaging” (see below).
Download an introduction and overview to the content of the Series chapters I-V.
Download the parts of the first chapter "Fundamental knowledge" below. |
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 Download introduction and overview (239 KB) |
Digital Material Analysis - Fundamental Knowledge
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Part 1 (General)
Physical principals of light and its interaction with optical surfaces. Consideration of such topics as wavelength, frequency and energy as well absorption, scatter, refraction, reflection, interference, polarisation and diffraction.
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| Download Part 1 (217 KB) |
Digital Material Analysis - Fundamental Knowledge
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Part 2 - Optical Terminology
Closer look at how underlying principles (see part 1) manifest in the capabilities and limitations of microscope optical systems. |
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| Download Part 2 (387 KB) |
Digital Material Analysis - Fundamental Knowledge
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Part 3 - From Theory to Reality
Take a look how the principles of Part 1 and 2 are put into practice – essentially the reality of microscopy for industrial applications is adressed. |
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| Download Part 3 (353 KB) |
Digital Material Analysis - Fundamental Knowledge
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Part 4 - Digitial Imaging Cameras: Deciphering Specifications
Look at the surprisingly complex world of digital microscopy cameras and learn how to find the best one for each research task. |
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| Download Part 4 (161 KB) |
Basics of light microscopy & imaging - Chapters I-V
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Basics of Light Microscopy & Imaging
All the chapters, the preface and the authors' CV's in one comprehensive volume. Downloads of single chapters I-V see below.
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| Download full article (6674 KB) |
Basics of light microscopy & imaging - Chapter I
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The Meaning of Colours for Microscopy and Imaging
Starting with the topic “colours”, this article will take you through the basics
of physical necessities and their implications for microscopy. |
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| Download chapter I (968 KB) |
Basics of light microscopy & imaging - Chapter II
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The Resolving Power
Experts at Olympus and Olympus Soft Imaging Solutions (OSIS) explain the theory and practice of resolution and Numerical Aperture, and how to optimise your system set up for observation, and for digital imaging.
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| Download chapter II (634 KB) |
Basics of light microscopy & imaging - Chapter III
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Contrast & Microscopy
The best contrast is essential for image quality, whether observed directly through the microscope or during digital image processing. Here we will highlight the practical features of different contrast techniques. |
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| Download chapter III (1209 KB) |
Basics of light microscopy & imaging - Chapter IV
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Fluorescence Microscopy
In this part of our “Basics of Light Microscopy and Imaging” series by experts from Olympus and Olympus Soft Imaging Solutions (former SIS), we will be concentrating on fluorescence microscopy and the essential role digital imaging plays. |
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| Download chapter IV (542 KB) |
Basics of light microscopy & imaging - Chapter V
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3D Imaging
This chapter explains the basics of 3D perception and introduces current techniques utilised to capture, record and display 3D views of microscopic samples. |
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| Download chapter V (781 KB) |
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