Specifications for scan^R |
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 Modular and flexible screening station
Fast and precise image acquisition and
analysis
Robust automated object detection and
cell separation
Visual correlation of cell images and
analysis results |
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Hardware
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Olympus IX81 microscope |
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CCD Hamamatsu: ORCA-ERG, C8484, ORCA 285. Intensified and EMCCD cameras (Hamamatsu) on request |
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Motorised Stage Märzhäuser SCAN IM IX2 |
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Imaging Computer (latest generation PC), 2 Hard-Drives (80 GB and 250 GB), 2GB RAM |
Hardware control
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Control board with additional CPU, independent from imaging PC |
Real-time Controller |
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Temporal resolution |
1 ms |
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Timing precision |
< 0.01 ms |
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Camera control |
trigger (level trigger if accepted by camera) |
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Peripheral device control via TTL pulses |
3 BNC connectors |
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Multi-task acquisition with hardware switch (z-position, exciter filter etc.), 50 ms exposure |
8 full frames/sec with parallel hardware switch |
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Settings can be saved as assays |
Illumination system
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MT20 |
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Short arc burners, |
150 W Xenon or Mercury-Xenon |
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8 Filter positions |
diameter 25 mm |
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Filter switch |
min. 58 ms (neighbouring positions) |
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Attenuation |
14 levels, 1% - 100% |
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Attenuation switch |
<58 ms |
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Shutter, on/off time |
1 ms |
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Operation |
all modules in parallel |
Image Acquisition
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Workflow oriented configuration and user interface |
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Variable powerful software auto-focus procedures |
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Format Manager with predefined formats (slides, multiwell plates) and editing interface to create and edit customised formats (spotted arrays) |
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On-line display |
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Fully automated operation |
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User interaction: pause, resume, set marker |
Image Analysis
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On-line & off-line analysis |
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Independent software module |
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Image processing |
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Image analysis & particle detection |
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Parameter extraction and calculation |
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Cytometric data analysis, gating & classification |
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Direkt link between data-points, objects and images |
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Data-export |
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Complex analysis procedures can be saved as assays |
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Predifined assays and advanced scientific assay development functionality |
Options
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IR-laser hardware autofocus based on ZDC |
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Plate-loading robot Hamilton MicroLab SWAP |
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Open or closed system bench |
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Customisation: Hardware, software, assays |
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Service contract |
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2nd licence scan^R analysis software |
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cell^cubator |
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Development version with LabVIEW source code interfaces |
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Fast filter turret |
min. switching time 350 ms |
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Professional data storage systems |
Performance
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Image acquisition 2 colour channels á 200 ms |
1 s/position |
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IR-hardware auto-focus |
1 - 2 s/position |
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software auto-focus |
2 - 5 s/position |
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24/7 tested |
General Features
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Maximum throughput and minimised bleaching/photo-toxicity due to real-time synchronisation |
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Homogeneous and high intensity fluorescence illumination by optimised illumination optics |
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Thermal insulation of samples and vibration free image acquisition by fiber-optic illumination coupling |
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High precision and high endurance components |
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Maximum flexibility and modularity by open microscope based system platform |
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Fluorescence and transmitted light screening |
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"Unlimited" colour channels |
Assays and Assay Development
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A powerful assay editor that is provided with the software allows to adapt the system to almost any assay |
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Example Assays
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Cell counting, Gene expression, Intracellular transport, Translocation, Cell proliferation, Promyelocytic leukemia (PML) body assay, Bacterial infection assay, Cell cycle analysis, Protein localisation and co-localisation, Live cell assays, Multi-colour assays, Rare event analysis, Automated FISH analysis, Fluorescence analysis in tissue sections, Cell array screens, Micronuclei and comet assays |
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