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Multiple Colour Fluorescence
cell^F controls of the acquisition and processing of multicoloured fluorescence images. It operates the motorised microscope, focus drive and camera. This means that it eg. controls the filter positions, the microscope objectives and the acquisition options of the individual fluorescences beforehand as well as camera settings such as exposure time, binning, number of integrations and of images. cell^F superimposes an unlimited number of single fluorescences (b/w or colour images) creating one multichannel combination image. Number and type of colours are selected according to the user’s wishes. A DIC or phase-contrast image with the emphasis desired can be superimposed onto the combination image. |
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Shift correction
Some filter combinations create small shifts in the image. This causes identical details to be shifted when imaged through different filters. cell^F includes a special built-in function to overcome these effects. The pixel-shift correction is applied automatically after an initial setup for each filter set. The final image contains all details with the selected emphasis and eliminates errors due to pixel shifts. |
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Spectral unmixing
Overlapping spectral characteristics and fluorescence “bleed-through“ into the “wrong“ colour channel limit the full exploitation of all the numerous fluorescent dyes and ever increasing family of fluorescent proteins in multi-labelled specimens. Linear spectral unmixing results in enhanced chromatic resolution and allows the simultaneous imaging of previously hardly useful fluorochrome combinations: for example GFP alongside YFP. The spectral redistribution maintains the overall intensity and facilitates quantitative analyses, for example in co-localization and FRET studies.
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Co-Localisation
cell^F supports the presentation and evaluation of co-localization. You can create an image showing only the overlapping areas between different fluorescence images. All evaluation data are displayed in a separate sheet. |
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Fast Fourier Transformation
Two-dimensional Fast Fourier Transformation (FFT) including convolution and correlation. In FFT space, the real and imaginary parts and the phase are supported. Annular, blob, lattice and sector filters are available and they can be logically connected. Specific frequencies or frequency ranges of the orientation can be shown/hidden in the actual image. |
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MT10_D illumination system control
cell^F controlls the multi-function all-in-one illumination system MT10_D. The illumination system MT10_D offers a fast switch of excitation wavelength. The integrated attenuator offers even faster switching and 7 grades of illumination intensity between 1% and 100%. The built-in shutter has an on/off time of less than 5 ms to efficiently reduce off-acquisition photobleaching. The light is coupled via a single quartz light fibre into the microscope using specially designed epi-fluorescence condensers for critical illumination of the specimen with maximum brightness and homogeneity. |
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WellNavigation
The WellNavigator application of cell^F supports the simple and fully automatic scan of standardised microtiter plates (eg 96 or 384). Integrated calibration routines for off-set and slant correction of the microtiter plates together with the integrated motorised stage control guarantee exact alignment and positioning. The software includes an Experiment Manager to easily define complex work processes. These are established
just once and then executed via the push of a button. Process series include complete microtiter plates, single wells or even clusters of wells. |
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Intuitive application control
Thanks to its intuitive user control, the 3.0 Workflow Assistant allows routine documentation and imaging tasks to be accomplished with just a few logical mouse clicks. |
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Olympus Camera Control
The Olympus Camera Control (OCC) tool window provides an intuitive and flexible control for all aspects of acquisition – from the Olympus cameras to the software. With OCC you can display and access the controls that you need and store and retrieve your application-specific camera settings. Simple, clear and precise! |
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Olympus True Color
Olympus True Colour (OTC) technology gives you the real image – from the specimen through the PC to the monitor. OTC uses internal ICC profiles to govern the relationship between the input colours from the specimen and through the camera, with the output colours from a display device, such as a monitor or printer. |
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Enhanced filter methods
